Effect of Protamine Coated PLA Nanocapsule on the Immune Function of Murine BMDCs In Vitro

VernacularTitle:鱼精蛋白包覆PLA纳米囊对小鼠骨髓系树突状细胞免疫功能的影响?
Author: Ping YANG ; Junming ZHU
Publication Type:Journal Article
Keywords: polylactic acid(PLA); protamine; nanocapsule; bone marrow-derived dendritic cell(BMDC); immune function
From: Acta Medicinae Universitatis Scientiae et Technologiae Huazhong 2016;45(3):298-301
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect of protamine(PS)coated polylactic acid(PLA)nanocapsule on the immune response of murine bone marrow-derived dendritic cells(BMDCs).Methods PLA nanocapsules were prepared by W1/O/W2 method.Protamine was used to modify the surface of PLA nanocapsule that encapsulated ovalbumin(OVA),a model anti-gen.The prepared OVA/PLA/PS nanocapsules were used to stimulate BMDCs.Flow cytometry was used to analyze the ex-pression of surface molecules CD80,CD83,CD86,MHCⅠ,and MHC Ⅱ,and the uptake of nanocapsule.The levels of IL-12 p70 secreted by BMDCs were detected by ELISA.Results OVA/PLA/PS nanocapsules could significantly up-regulate the expres-sion of CD80,CD83,MHCⅠ,and MHC Ⅱ in BMDCs,and increase the secretion of IL-12 p70 by BMDCs.Furthermore,OVA/PLA/PS nanocapsules could enhance the uptake efficiency of OVA by BMDCs.Conclusion OVA/PLA/PS nanocapsule can en-hance the immune response of BMDCs,and may become a good drug delivery carrier.