Inhibitory effect of baicalin on invasion of cervical cancer HeLa cells and its mechanism
10.7652/jdyxb201604027
- VernacularTitle:黄芩苷对宫颈癌 He La细胞侵袭转移的抑制作用及其机制
- Author:
Yue ZHANG
;
Qiaoshan FU
;
Wei LIU
;
Qing GAO
- Publication Type:Journal Article
- Keywords:
baicalin;
HeLa cell;
invasion;
P38 signal pathway;
matrix metalloproteinases (MMP)
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2016;37(4):599-603
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the anti‐metastatic effect and mechanism of baicalin on the growth of HeLa cells was measured by MTT assay ,and cell migration baicalin on human cervical cancer HeLa cells .Methods The effects of baicalin on the proliferation and invasion of HeLa cells were analyzed by MTT method and Transwell assay .Moreover ,Real‐time PCR was used for investigating the expressions of MMP‐2 and MMP‐9 at the RNA level .Western blot was used for investigating the expressions of MMP‐2 ,MMP‐9 ,P38 and p‐P38 at the protein level .Results Baicalin could significantly inhibit the proliferation of HeLa cells in the dose‐dependent manner at the concentration above 60 μg/mL . Anti‐metastatic signaling induced by baicalin was characterized by down‐regulating the RNA and protein expressions of MMP‐2 and MMP‐9 ,and down‐regulating the phosphorylation level of P38 . Pre‐treatment of P38 signal pathway inhibitor could enhance the inhibitory effect of baicalin on the expressions of MMP‐2 and MMP‐9 .Conclusion These results indicate that baicalin‐induced anti‐metastatic effect involves the inhibition of MMP‐2 and MMP‐9 in HeLa cells through P38 signal pathway .
