Effects of vIL-10 on MHC-I antigen processing“the operon”in nasopharyngeal carcinoma cell lines
10.7652/jdyxb201604011
- VernacularTitle:vIL-10对鼻咽癌细胞株 MHC-1类分子抗原加工提呈“操纵子”表达的影响
- Author:
Yanxin REN
;
Jie YANG
;
Ruimei SUN
;
Liufang ZHAO
;
Lei LI
;
Shiwen ZHANG
;
Jimin FEI
;
Yitai SHANG
;
Zhoulei LI
;
Xiaojiang LI
- Publication Type:Journal Article
- Keywords:
nasopharyngeal carcinoma (NPC);
virus interleukin-10;
transporter associated with antigen pro-cessing (TAP);
low molecular mass polypeptide (LMP);
human leukocyte antigen I (HLA-I)
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2016;37(4):525-530
- CountryChina
- Language:Chinese
-
Abstract:
ABSTRACT:Objective To explore the effects of virus interleukin‐10 (vIL‐10 ) on different expressions of MHC‐I antigen processing “the operon” .Methods We collected nasopharyngeal carcinoma cells (CNE‐1 and CNE‐2) treated by vIL‐10 at different time points ,and detected the changes of MHC‐I antigen processing “the operons” (TAP‐1 ,TAP‐2 ,LMP‐2 ,LMP‐7 and HLA‐I) by RT‐PCR and Western blot .Results ① mRNA level :There was no difference in the expression of TAP‐1 in CNE‐1 and CNE‐2 cells at various time points .The expressions of TAP‐2 and LMP‐2 in CNE‐1 and CNE‐2 did not change at 1 ,4 ,6 ,12 h ,but downregulated and even disappeared at 24 h .The expression of LMP‐7 in CNE‐1 decreased 4 h after vIL‐10 was added ,and that in CNE‐2 decreased at 6 h .The expression of HLA‐I in CNE‐1 and CNE‐2 showed significant decrease at 24 h .② Protein expression :The expression of TAP‐1 in CNE‐1 and CNE‐2 showed significant decrease at 24 h .The expression of TAP‐2 in CNE‐1 and CNE‐2 was gradually downregulated at different time points .The expressions of LMP‐2 and LMP‐7 in CNE‐2 were gradually downregulated at different periods ,while that in CNE‐1 was only decreased at 12 h .The expression of HLA‐I in CNE‐1 and CNE‐2 was gradually downregulated ,but there was no significant difference at each period in CNE‐1 ,while the expression of HLA‐I in CNE‐2 at 24 h was significantly downregulated .Conclusion vIL‐10 can inhibit the expression of MHC‐I antigen processing “the operon” in NPC in the time‐dependent manner .
