Application of white blood cell differential in peripheral blood by flow cytometry
10.3760/cma.j.issn.1009-9158.2016.05.014
- VernacularTitle:流式细胞术在外周血白细胞分类计数中的应用
- Author:
Chenxue QU
;
Xuelian WU
;
Juhua DAI
;
Liping LI
- Publication Type:Journal Article
- Keywords:
Leukocyte;
Differential count;
Flow cytometry
- From:
Chinese Journal of Laboratory Medicine
2016;39(5):389-392
- CountryChina
- Language:Chinese
-
Abstract:
Manual microscopic differential of white blood cell has been challenged by multiparameter flow cytometry,using monoclonal antibodies to define the different leukocyte types.Compared with manual differential,flow cytometry method is more sensitive,specific,objective and has good repeatability.Recent studies demonstrated flow cytometric differential correlates well with manual microscopic method and has good clinical performance for blast and immature granulocyte.Meanwhile more leukocyte populations can be identified with flow cytometric method,such as lymphocyte subset and CD 16 + monocyte,thus helping in monitoring blast in acute leukemia,B lymphocyte proliferative disorder differential diagnosis and minimal residual disease.With the development and improvement of flow cytometric differential,it might be a candidate reference method of leukocyte differential,gradually applied in the routine work.
