The effect of HIV infection and HAART on mitochondrion in peripheral monocytes
10.3760/cma.j.issn.1009-9158.2016.05.010
- VernacularTitle:HIV感染和高效联合抗病毒治疗对外周血单核细胞线粒体的影响
- Author:
Fengting YU
;
Yu HAO
;
Hongxin ZHAO
;
Fujie ZHANG
- Publication Type:Journal Article
- Keywords:
HIV infections;
Antiretroviral therapy,highly active;
Monocytes;
Membrane potential,mitochondria
- From:
Chinese Journal of Laboratory Medicine
2016;39(5):372-375
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of HIV infection and highly active antiretroviral therapy (HAART) on mitochondrial function and mass in peripheral monocytes.Methods There were 14 ART-naive HIV-infected patients,14 NRTI treated HIV-infected patients and 12 healthy controls from Beijing Ditan Hospital.The mitochondrial membrane potential and mitochondrial mass in monocytes were analyzed by flow cytometry.Mitochondrial disturbances related to HIV infection and HAART in monocytes were analyzed.Results In ART-naive patients and NRTI-exposed patients,the levels of mitochondrial membrane potential in monocytes (77.74 ± 14.77,73.94 ± 12.87) were significantly lower than these in healthy controls (89.43 ±4.06) (P =0.032 8,P =0.002 6).The amount of mitochondrial mass in NRTI-exposed patients (3 329.0 ± 836.7) was significantly higher than that in healthy controls (2 075.0 ± 932.2) and that in ART-naive patients (2 592.0 ± 781.5) (P ＜ 0.05).Conclusions The abnormal of mitochondrial membrane potential and mitochondrial mass in monocytes from HIV-infected patients were related to HIV infection and the introduction of HAART.
