A study of the lipoprotein lipase inhibitory mechanism of Poncirus trifoliata water extracts.
- Author:
Sung Mee LEE
1
;
Yun Hwan KANG
;
Kyoung Kon KIM
;
Tae Woo KIM
;
Myeon CHOE
Author Information
- Publication Type:Original Article
- Keywords: Poncirus trifoliata; lipoprotein lipase; sortilin-related receptor; CCAAT-enhancer-binding proteins (C/EBP) beta; anti-obesity
- MeSH: Adipocytes; Biomarkers; Blotting, Western; CCAAT-Enhancer-Binding Proteins; Cell Fractionation; Endocytosis; Enzyme-Linked Immunosorbent Assay; Fruit; Lipoprotein Lipase*; Polymerase Chain Reaction; Poncirus*; Protein Transport; Reverse Transcription; RNA, Messenger; Transcription Factors; Water*
- From:Journal of Nutrition and Health 2015;48(1):9-18
- CountryRepublic of Korea
- Language:Korean
- Abstract: PURPOSE: Poncirus trifoliata has been reported to have anti-inflammatory, antioxidant, and immune activities. However, its anti-obesity activity and the mechanism by which the water extract of dried, immature fruit of Poncirus trifoliata (PF-W) acts are not clear. This study suggests a potential mechanism associated with the anti-obesity activity of PF-W. METHODS: We measured the effect of PF-W on lipoprotein lipase (LPL) regulation using enzyme-linked immunosorbent assay (ELISA) and an activity assay. The LPL regulation mechanism was examined by reverse transcription polymerase chain reaction (RT-PCR) to measure the mRNA expression of biomarkers related to protein transport and by western blot for analysis of the protein expression of the transcription factor CCAAT-enhancer-binding protein (C/EBPbeta) RESULTS: The total polyphenol and flavonoid content of PF-W was 52.15 +/- 4.02 and 6.56 +/- 0.47 mg/g, respectively. PF-W treatment decreased LPL content in media to 58 +/- 5% of that in control adipocyte media, and increased LPL content to 117 +/- 3.5% of that in control adipocytes, but did not affect the mRNA expression of LPL. PF-W also increased the mRNA expression of sortilin-related receptor (SorLA), a receptor that induces endocytosis and intracellular trafficking of LPL, in a concentration- and time-dependent manner. Finally, cell fractionation revealed that PF-W treatment induced the expression of C/EBPbeta, a SorLA transcription factor, in the nuclei of 3T3-L1 adipocytes. CONCLUSION: The LPL secretion and activity assay showed PF-W to be an LPL secretion inhibitor, and these results suggest the potential mechanism of PF-W involving inhibition of LPL secretion through C/EBPbeta-mediated induction of SorLA expression.
