Effects of Extract of Ginkgo biloba Leaves EGb761 on MPP+-induced Injury in SH-SY5Y Cells
10.3969/j.issn.1005-5304.2016.07.018
- VernacularTitle:银杏叶提取物EGb761对MPP+诱导的人脑神经瘤母细胞SH-SY5Y细胞损伤的影响
- Author:
Lihua XUE
;
Xingbin WU
;
Foyan LIAN
;
Jian PAN
- Publication Type:Journal Article
- Keywords:
extract of Ginkgo biloba leaves EGb761;
SH-SY5Y cell;
Parkinson’s disease;
NO free radical
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2016;23(7):70-73
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of extract of Ginkgo biloba leaves EGb761 on 1-methy-l 4-phenylpyridium (MPP+)-induced injury in human neuroblastoma SH-SY5Y cells; To discuss its mechanism of action.Methods Cell culture method was used and SH-SY5Y cell damage model was induced with different concentrations of MPP+ to build Parkinson’s disease model in vitro. The experiment was divided into control group, MPP+ model group, low-, medium-, and high-dose EGb761 groups. The survival rate was determined by MTT assay, and the apoptotic rate was detected by flow cytometry according to AnnexinV apoptosis detection kit. The cell morphology was observed by inverted microscope. NO content in SH-SY5Y cells was detected by Nitric acid reduction method.Results Compared with the control group, the survival rate of SH-SY5Y cells decreased and the apoptotic rate and NO content increased in the model group (P<0.05); Compared with the model group, the survival rate of SH-SY5Y cells increased and the apoptotic rate and NO content decreased in the low-, medium- and high-dose EGb761 groups (P<0.05).Conclusion EGb761 can protect MPP+-induced SH-SY5Y cell from damage by the inhibition of the content of NO free radical.
