HPLC Wavelength Switching Simultaneous Determination for the Contents of Mangiferin and Hesperidin inYinhua Mangguo Tablets
10.3969/j.issn.1005-5304.2016.07.025
- VernacularTitle:HPLC波长切换法测定银花芒果片中芒果苷、橙皮苷含量
- Author:
Dezhi TANG
- Publication Type:Journal Article
- Keywords:
Yinhua Mangguo Tablets;
mangiferin;
hesperidin;
wavelength switching
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2016;23(7):98-100
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an HPLC method of wavelength switching simultaneous determination for the contents of mangiferin and hesperidin inYinhua Mangguo Tablets.Methods Agilent ZORBAX Eclipse XDB-C18 (4.6 mm×150 mm, 5 μm) column was used. The mobile phase was methanol-water containing 0.2% phosphoric acid with gradient elution mode at the flow rate of 1.0 mL/min; the detection wavelength was 258 nm (0–20 min) for mangiferin, 283 nm (20–50 min) for hesperidin.Results Mangiferin had a good linearity in the range of 0.042–0.761 μg, and the average recovery was 99.86%, with RSD of 1.5% (n=9). Hesperidin had a good linearity in the range of 0.076–1.361 μg, and the average recovery was 99.91%, with RSD of 0.7% (n=9).Conclusion The method is simple, accurate and reproducible, which can be used to the quality control ofYinhua Mangguo Tablets.
