Content Determination of Five Nucleosides in Hedyotis Diffusa and Its Adulterants by UPLC
10.3969/j.issn.1005-5304.2016.07.023
- VernacularTitle:白花蛇舌草及其混淆品中5种抗肿瘤核苷类成分含量测定
- Author:
Xinfeng WANG
;
Chuanjiang MA
;
Guangshang CAO
- Publication Type:Journal Article
- Keywords:
Hedyotis diffusa;
content determination;
nucleosides;
UPLC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2016;23(7):92-94
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore an UPLC method for simultaneous content determination of the five nucleosides (cytidine, uridine, adenine, thymidine and adenosine) in Hedyotis diffusa and its adulterants; To compare the content differences.Methods The analysis was performed on a BEH C18 column (2.1 mm×50 mm, 1.7 μm) by UPLC eluted with acetonitrile and water in gradient mode. The flow rate was 0.5 mL/min; the detection wavelength was set at 254 nm; the column temperature was set at 30℃.Results Five nucleosides have good linear relationship, precision, stability, and repeatability according to the requirements of the methodology determination. The recoveries were among 98.7%–101.5%. Five nucleosides in Hedyotis diffusa and its adulterants from different areas were determined by the UPLC method.Conclusion The method is certified to be simple, rapid, accurate and reliable, which can be used for the determination of nucleosides in Hedyotis diffusa and its adulterants.
