Determination of Aspartame in Xiaoer Anfen Huang Namin Granule by HPLC
- VernacularTitle:HPLC 法测定小儿氨酚黄那敏颗粒中阿斯帕坦的含量
- Author:
Fei SUN
- Publication Type:Journal Article
- Keywords:
Xiaoer Anfen Huang Namin granule;
Aspartame;
Determination;
HPLC
- From:
China Pharmacist
2016;19(5):1013-1015
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an HPLC method for the determination of aspartame in Xiaoer Anfen Huang Namin granule. Methods:An Agilent Zorbax SB-C18 column(250 mm × 4. 6 mm,5 μm)was used with the mobile phase of acetonitrile-0. 05 mol·L -1 H3 PO4(20 ∶80). The flow rate was 1. 0 ml ·min -1 and the detection wavelength was 204 nm. The column temperature was 25℃ and the injection volume was 10 μl. Results:Aspartame had a good linear relationship within the range of 21. 72- 868. 67 μg · ml -1(r = 1. 000 0). The average recovery of aspartame was 99. 36% and RSD was 0. 3% (n = 6). Conclusion:The method has good stability and repeatability,and is suitable for the quality control of Xiaoer Anfen Huang Namin granule.
