Determination of Ethyl Pyruvate by GC
- VernacularTitle:GC 法测定丙酮酸乙酯的含量
- Author:
Xiaoyun WANG
;
Fayan MAO
;
Xiaogang WANG
;
Yanru HUANG
;
Chunxu HAI
;
Peng LIU
;
Wei HE
- Publication Type:Journal Article
- Keywords:
Ethyl pyruvate;
GC;
Determination
- From:
China Pharmacist
2016;19(5):1021-1023
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method for the determination of ethyl pyruvate by GC. Methods:A Varian CP7502 capillary column(25 m × 0. 25 mm,0. 25 μm)was used. The carrier gas was nitrogen with the flow rate of 30 ml·min-1 ,the gas was hydrogen with the flow rate of 40 ml·min -1 and the oxidant gas was air with the flow rate of 400 ml·min-1 . The detector was an FID and the inlet temperature was 210℃ . The temperature program was as follows:the initial column temperature was 40℃, and then risen to 200℃ with a rate of 15 ℃·min -1 . The split ratio was 100 ∶1 and the injection volume was 1μl. Results:Ethyl pyruvate had a good linear relationship within the range of 0. 025 6- 8. 192 8 mg·ml -1 ,and r was 0. 999 8. The average recovery was99. 99% and RSD was 1. 38%(n = 9). Conclusion:The method is accurate,simple and rapid with good stability and high sensitivity,which can be used to determine the content of ethyl pyruvate.
