The Activation and Polarization of Microglia in Epileptic Rats Induced by Pilocarpine
- VernacularTitle:匹罗卡品诱导癫痫大鼠模型小胶质细胞的激活与极化
- Author:
Lianmei ZHONG
;
Qinglong AI
;
Jiazhi GUO
;
Jun SUN
;
Di LU
;
Yanfang WU
;
Ligong BIAN
;
Zhirong ZOU
- Publication Type:Journal Article
- Keywords:
Microglia;
Epilepsy;
Rat;
iNOS;
Arg-1
- From:
Journal of Kunming Medical University
2016;37(5):1-4
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the activition and polarization of microglia in the epileptic rats induced by lithium chloride-pilocarpine. Methods One hundred male SD rats were randomly divided into five groups: control group and different time points model groups including 1d,3d,7d and 14d. Epilepsy models were established by lithium chloride-pilocarpine intraperitoneal injection. The control group was given the same dosage of normal saline. The morphology change was detected by immunofluorescence,and the expressions of iNOS and Arg-1 were determined by IHC at respective time points. Results Compared the model groups with control group,microglia was activated,synapsis was shorten,volume got bigger,most of them seemed as amoebocyte,the expression of iNOS increased and Arg-1 decreased,especially at 3d.ConclusionThe results from this study indicated that microglia was activated and polarized in epileptic rats induced by pilocarpine.
