Anti-tumor Effects of Total Saponins Extract from the Aerial and Underground Parts ofParispolyphyllavar.yunnanensis
- VernacularTitle:重楼地上部分与地下部分皂苷的抗肿瘤作用
- Author:
Ju LI
;
Rongping ZHANG
;
Zhiyong HE
;
Shuda YANG
;
Weiyan HU
- Publication Type:Journal Article
- Keywords:
Total saponin;
Underground part of Parispolyphylla var.yunnanensis;
Aboveground part of Parispolyphyllavar.yunnanensis
- From:
Journal of Kunming Medical University
2016;37(5):46-50
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the anti-tumor effects of total saponins,extracted from stems and leaves ofParispolyphyllavar.yunnanensis. Methods Taking mouse stomach carcinoma MFC cell line,human mammary cancer cell line(MCF-7)and human cervical carcinoma cell line(Hela),and their tumor-bearing mice as models,the antitumor activity was carried out in vitro and in vivo. CCK-8 assay was used to determine the inhibitory effect in vitro. The tumor-bearing mice model was induced by tumor cell vaccination in normal mouse forelimb left axillary subcutaneous and these mice were randomized into NS group,cytoxan group(30 mg/kg),saponins high-dose,medium-dose and low-dose groups(60,30 and 15 mg/kg). They were given intraperitoneal injection once a day for consecutive 15 days. The tumor inhibition rate and survival of the tumor-bearing mice were measured. Results Saponins,extracted from both aboveground and underground ofP.polyphyllavar.yunnanensis could significantly inhibit the growth of MFC,MCF-7 and Hela cells in time- and dose-dependent manners. The tumor weight in each drug treated group was significantly lower than that in the control group. Conclusion Saponins,extracted from both aboveground and underground ofP.polyphyllavar.yunnanensis have antitumor activity.
