Proliferation of Porcine Retinal Pigment Epithelium after Laser Photocoagulation in Organ Culture.
- Author:
Hyeon Sook KIM
1
;
Nam Chun CHO
;
Hong Joo HAN
Author Information
1. Department of Ophthalmology, College of Medicine, Chonbuk National University, Chonju, Korea.
- Publication Type:In Vitro ; Original Article
- Keywords:
ABGL;
Organ culture;
Proliferation;
Laser power;
RPE
- MeSH:
Argon;
Bruch Membrane;
Choroid;
Light Coagulation*;
Organ Culture Techniques*;
Retinal Pigment Epithelium*;
Retinaldehyde*;
Sclera
- From:Journal of the Korean Ophthalmological Society
1995;36(2):240-246
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Laser photocoagulation was applied in vitro to organ culture exoplants of porcine retinal pigment epithelium(RPE) attached to Bruch's membrane, choroid, and sclera using the Del Priore method for de-monstrating the proliferation of RPE cells and characterizing the response with respect to power level of treatment. Six-millimetor-round buttons of eyewall were treated with 20-30 spots from the argon bluegreen laser using a 500 micrometer spot size, 0.1s duration, and variable powers(100mW, 300mW, and 500mW). Group 1 is untreated control group and group 2(100mW) and group 4(500mW) showed less active proliferation of RPE than group 3(300mW). In group 3, all RPE cells, Bruch's membrane, and a part of choroid were disrupted and lifted off three hours after laser photocoagulation and then RPE cells began to proliferate actively at third day. The treated area became completely covered with several layers of RPE cells. The proliferation of RPE cells turned out to be larger when the power was moderate(300mW) as compared to the case when the power was too high(500mW) or too low(100mW).
