Genetic Diversity Analysis of Desmodium styracifolium from Different Habitats by ISSR Analysis
- VernacularTitle:不同地区广金钱草遗传多样性的 ISSR 分析
- Author:
Bo HE
;
Yong XU
;
Min YANG
;
Keli CHEN
;
Bisheng HUANG
- Publication Type:Journal Article
- Keywords:
Desmodium styraei folium(Osb.)Merr.;
ISSR;
Genetic diversity
- From:
China Pharmacist
2016;19(5):884-887
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To assess the genetic diversity and phylogenetic relationship of Desmodium styraeifolium from fifteen regions of Guangdong and Guangxi Zhuang autonomous region. Methods:The molecular technique ISSR((inter-simple sequence repeat))was applied to investigate the genetic diversity of Desmodium styraeifolium from fifteen regions of Guangdong and Guangxi Zhuang autonomous region. The data was analyzed with Popgene 1. 32,and a cluster diagram was presented by UPGMA. Results:Totally 51 amplified fragments were obtained by 7 ISSR primers. The results analyzed by Popgene 1. 32 showed that the Shannon diversity index(I)was 0. 3,the NEI’s genetic diversity coefficient(H)was 0. 246 4,the coefficient of genetic differentiation (GST)was 0. 123 8,and the gene flow(Nm)was 3. 539 7. Conclusion:The above mentioned results exhibit that Desmodium styraeifolium from Guangdong,Guangxi and some wild herbal populations has high genetic diversity. The clustering results illustrate that the genetic distance of Desmodium styraeifolium originated from Guangdong and Guangxi is related with geographic distance.
