Determination of Strychnine and Brucine in Strychnos Nux-vomica Linn by SPME-HPLC
- VernacularTitle:固相微萃取-高效液相色谱联用测定马钱子中士的宁、马钱子碱含量
- Author:
Qin GENG
;
Li WANG
- Publication Type:Journal Article
- Keywords:
Stryehnos nux-vomiea Linn;
Strychnine;
Brucine;
SPME-HPLC
- From:
China Pharmacist
2016;19(5):994-996
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a SPME-HPLC method for the determination of strychnine and brucine in Stryehnos nux-vomiea Linn. Methods:A novel capillary microtube monolithic column coupled with HPLC was prepared for the selective solid-phase microextraction of strychnine and brucine in Stryehnos nux-vomiea Linn. A Hypersil ODS column(150 mm × 4. 6 mm, 5 μm)was used and the mobile phase was acetonitrile- 0. 01 mol·L -1 sodium heptanesulfonate mixed with the same quantity of 0. 02 mol · L -1 potassium dihydrogen phosphate(adjusting pH to 2. 8 with 10% phosphoric acid)(21 ∶79). The detection wavelength was 260 nm,the sample size was 20 μl,and the column temperature was 25℃ . Results:The linear range of strychnine was 1. 2-90. 0μg·ml -1(r = 0. 999 8)and that of brucine was 1. 0-75. 0μg·ml -1(r = 0. 999 6). The average recovery was 100. 05%(RSD = 0. 58% ,n = 6)for strychnine and 99. 98%(RSD = 0. 27% ,n = 6)for brucine. Conclusion:The method is accurate and highly efficient,which can be used for the determination of strychnine and brucine in Stryehnos nux-vomiea Linn.
