Determination of Fenbendazole and Praziquantel in Compound Fenbendazole Tablets by HPLC
- VernacularTitle:HPLC 法测定复方芬苯达唑片中芬苯达唑和吡喹酮的含量
- Author:
Yan DING
- Publication Type:Journal Article
- Keywords:
HPLC;
Fenbendazole;
Praziquantel;
Determination
- From:
China Pharmacist
2016;19(5):1011-1013
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To develop an HPLC method for the determination of fenbendazole and praziquantel in compound fenbendazole tablets. Methods:An InertSustain? C18 column(250 mm × 4. 6 mm,5 μm)was used. The mobile phase was acetonitrile-phosphate buffer(pH = 3)(48 ∶52)and the flow rate was 1. 0 ml·min-1 . The detection wavelength was 214 nm,the column temperature was 30 ℃ and the sample size was 10 μl. Results:The linear range of fenbendazole and praziquantel was 4. 545- 63. 630 μg·ml -1(r = 0. 999 9)and 0. 225- 3. 150 μg·ml-1(r = 0. 999 9),respectively. The average recovery was 101. 09%(RSD = 0. 28% ,n = 9)and 99. 47%(RSD = 0. 78% ,n = 9),respectively. Conclusion:The method is simple,fast, accurate and applicable in the determination of fenbendazole and praziquantel in compound fenbendazole tablets.
