Effects of arsenic trioxide combined with cisplatin on expression of PCNA and Ki-67 in human nasopharyngeal carcinoma xenograft in nude mice
10.16066/j.1672-7002.2016.02.009
- VernacularTitle:亚砷酸联合顺铂对人鼻咽癌裸鼠移植瘤及增殖细胞核抗原和Ki-67的影响
- Author:
Xueqin HUANG
;
Junli HU
;
Hui ZHOU
;
Xide CHEN
;
Yanyan WANG
;
Yuefei ZHANG
- Publication Type:Journal Article
- Keywords:
Cisplatin;
Nasopharyngeal Neoplasms;
Proliferating Cell Nuclear Antigen;
Immunohistochemistry;
arsenic trioxide;
nude mice
- From:
Chinese Archives of Otolaryngology-Head and Neck Surgery
2016;23(2):93-97
- CountryChina
- Language:Chinese
-
Abstract:
[ABSTRACT]OBJECTIVETo study the effects of Arsenic Trioxide on the growth of human nasopharyngeal carcinoma cell strain CNE-2Z xenograft in nude mice, and explore the possible mechanisms of the antitumor effect. METHODSThe models of human poorly differentiated nasopharyngeal carcinoma in nude mice were established and randomly divided into 4 groups (control group, As2O3 group, DDP group and As2O3+DDP group). The antitumor effect of every group was studied. Pathological changes of tumor tissues dyed by HE staining were observed with light microscope. The pathological changes of the heart, liver, lung and kidney from nude mice were also observed by HE staining. Blood routine test was examined. Expression of PCNA and Ki-67 were detected by immunohistochemistry method.RESULTSThe growth of tumor in As2O3, DDP and As2O3+DDP were obviously inhibited. No pathological changes and metastases were found in heart, liver, lung and kidney of the nude mice. No obvious toxicity of the treatment to the hematopoietic systems in the nude mice was observed. The expression of PCNA and Ki-67 were down-regulated.CONCLUSIONAs2O3 can inhibit the growth of human nasopharyngeal carcinoma cell strain CNE-2Z xenograft in nude mice, the mechanism of which might be related to the down-regulation of PCNA and Ki-67.
