IL-27 Alleviates the Bleomycin-induced Pulmonary Fibrosis through Regulating TGF-β/Smad Signaling Pathway
- VernacularTitle:IL-27调控T GF-β/Smad 通路在博来霉素诱导肺纤维化中的作用
- Author:
Wen LEI
;
Tao ZHANG
;
Xiaoyuan ZHAO
;
Ying WANG
;
Zhenkun LI
;
Zhaoxing DONG
- Publication Type:Journal Article
- Keywords:
IL-27;
Pulmonary fibrosisp;
TGF-β/Smad signaling pathway
- From:
Journal of Kunming Medical University
2016;37(5):9-12
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore whether IL-27 inhibited the pulmonary fibrosis through regulating the expression of TGF-β/Smad signaling pathway in the bleomycin-induced pulmonary fibrosis model. Methods Forty male C57/BL6 mice were randomly divided into normal control group(group A),bleomycin-induced pulmonary fibrosis group(group B),bleomycin+IL-27 group(group C)and bleomycin+IL-27 antibody group(group D) with 10 in each. Five mice in each group were sacrificed on days 7 and 28 after with intratracheal bleomycin. TGF-βR1,Smad1 and Smad3 in right lung tissue were measured by Western Blot. Results 1. In the bleomycin-induced pulmonary fibrosis model,the expression of TGF-βR1 was higher on days 7 and 28,which was inhibited by IL-27. 2. The expressions of p-Smad1 and p-Smad3 were highest in group D on days 7 and 28, but were lower in group C on day 7 than those in group B. Conclusion Exogenous IL-27 might alleviate pulmonary fibrosis through inhibiting the related protein phosphorylation in TGF-β/Smad signaling pathway.
