Plasmid and stable cell linereconstruction of RET point mutation in medullary thyroid carcinoma pedigree
10.16066/j.1672-7002.2016.05.002
- VernacularTitle:甲状腺髓样癌家系RET基因点突变质粒及稳定细胞系构建
- Author:
Fanqian LU
;
Xiaohong CHEN
;
Xiujuan KOU
;
Yunlong BAI
;
Yaru FENG
;
Jing YANG
;
Bochun WANG
- Publication Type:Journal Article
- Keywords:
Thyroid Neoplasms;
Genes;
Mutation;
Plasmids;
Lentivirus Infections;
Cell Line;
signaling pathway
- From:
Chinese Archives of Otolaryngology-Head and Neck Surgery
2016;23(5):250-252
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThe plasmid construction and validation of RET point mutation in vitro.METHODS The RET gene exon in the mainland familial medullary thyroid carcinoma family was analized with muon capture two generation sequencing of. In vitro, the RET relative point mutationswere reconstructed in NIH3T3 cells and the expression levels were studied.RESULTSThe corresponding lentiviral plasmids of RET point mutations were successfully obtained after point mutating the wild RET plasmids, it was verified that the target genes were expressed in NIH3T3 cells stably by Western Blot. CONCLUSIONSStable cell lines carrying RET point mutations were reconstructedsuccessfully, which provide a good platform for studying various point mutations.
