Effect of antisense CK13 genes on human nasopharyngeal carcinoma HNE1 cell transplantation tumor
10.16066/j.1672-7002.2016.05.003
- VernacularTitle:反义细胞角蛋白13基因对人鼻咽癌HNE1细胞移植瘤放疗敏感性的影响
- Author:
Chunyu MIAO
;
Hong YU
- Publication Type:Journal Article
- Keywords:
Nasopharyngeal Neoplasms;
Apoptosis;
Cell Cycle;
anti-cytokeratin 13
- From:
Chinese Archives of Otolaryngology-Head and Neck Surgery
2016;23(5):253-258
- CountryChina
- Language:Chinese
-
Abstract:
[ABSTRACT]OBJECTIVEThe influnence of observation on the antisense cytokeratin 13 (CK13) gene in nasopharyngeal carcinoma HNE1 cell transplantation tumor radiation sensitivity.METHODSHNE1 cell lines can be divided into control group: the control group (HNE1 cell) and lentivirus (transfection slow virus empty carrier) group and experimental group: HNE1-anti-CK13a (transfection antisense CK13a slow virus) and HNE1-anti-CK13b (transfection antisense slow virus CK13b) four groups, set up a corresponding animal model, after radiotherapy by flow cytometry, immunohistochemistry, PCR, Tunel method and Westernblotting detection.RESULTSThe cell cyclede tection of plasmid transfection slow virus group compared with control group after radiotherapy G2/M phase of the block were significantly prolonged; Immunohistochemical results showed emigration tumor CK13 expression decreased in the experimental group; Tunel method to detect apoptosis necrosis rate found that the experimental group,apoptosis rate significantly decreased; Western blotting detection caspase3 apoptosis markers. PCR to detect CDC25mRNA level decreased obviously.CONCLUSIONAntisense CK13 gene by regulating the cell cycle and apoptosis can reduce HNE1 transplantation tumor radiotherapy of nasopharyngeal carcinoma (NPC) sensitivity, and with the caspase 3 apoptotic pathways and CDC25 signaling pathways.
