The endogenous oxindole isatin induces apoptosis of MCF-7 breast cancer cells through a p53-mediated mitochondrial pathway
10.3969/j.issn.1001-1978.2016.06.008
- VernacularTitle:天然活性分子isatin经p53介导的线粒体途径诱导乳腺癌细胞MCF-7凋亡
- Author:
Jinlian SONG
;
Zhongliang MA
;
Xiaowei CHI
;
Yanping CHEN
;
Lin HOU
- Publication Type:Journal Article
- Keywords:
isatin;
human breast cancer cell;
apopto-sis;
p53;
Bcl-2;
Bax;
mitochondrial membrane poten-tial
- From:
Chinese Pharmacological Bulletin
2016;32(6):773-778
- CountryChina
- Language:Chinese
-
Abstract:
Aim Toinvestigatetheinducementeffect of isatin on apoptosis of breast cancer cell line MCF-7 , andexploreitsdetailedmechanism.Methods MCF-7 cell lines were exposed to isatin at different concentra-tions(0,50,100,200 μmol·L-1 )for 48 h.Apop-totic features were demonstrated by nuclei staining with Hoechst 33258.Bcl-2,Bax and p53 mRNA were ana-lyzed by RT-PCR.Caspase-9 activation and mitochon-drial depolarization were assayed by flow cytometry. Bcl-2,Bax,p53 and cytochrome c proteins were ana-lyzedbyWesternblot.Results Isatininducesapopto-sis of MCF-7 cells.Furthermore,Bcl-2 expression was decreased and the ratio of Bcl-2 to Bax was significant-ly decreased by isatin.While,p53 expression relative-ly decreased.The mitochondrial transmembrane poten-tial was markedly reduced and the release of cyto-chrome c into the cytosol was increased after treatment with isatin.Simultaneously,caspase-9 was activated. Conclusions Isatinsignificantlyinducedtheapopto-sis of MCF-7 cells in vitro.These results strongly sug-gest that the p53 dependent mitochondrial pathway is involved in apoptosis.
