Acteoside promotes proliferation of neural stem cells from adult mice by activating PI3 K/AKT pathway
10.3969/j.issn.1001-1978.2016.06.019
- VernacularTitle:毛蕊花糖苷通过激活PI3 K／AKT通路促进成年小鼠神经干细胞增殖
- Author:
Huimin LIN
;
Weibing DUAN
;
Rui SHAO
;
Lifeng HAN
;
Yan ZHU
;
Xiumei GAO
;
Yu WANG
- Publication Type:Journal Article
- Keywords:
acteoside;
adult mice;
neural stem cells (NSCs);
the subventricular zone(SVZ);
proliferation;
Akt pathway
- From:
Chinese Pharmacological Bulletin
2016;32(6):836-840
- CountryChina
- Language:Chinese
-
Abstract:
Aim Toclarifytheeffectofacteosideon proliferation of neural stem cells (NSCs ) from adult mice,as well as the involved signaling pathway.Meth-ods NSCswereisolatedfromthesubventricularzone (SVZ)of adult C57BL/6 mice,then identified by im-munofluorescence staining with Nestin,the marker of NSCs.NSCs were exposed to acteoside (5,10,20,40μmol·L-1 )in absence of mitogen(EGF/bFGF)for 24 h.We employed CCK8 assay to detect NSCs viability and BrdU staining to identify NSCs proliferation.We performed Western blot to quantify the expression level ofp-AktinducedbyacteosideonNSCs.Results With-out mitogen,acteoside increased NSCs proliferation by activating p-Akt,which can be blocked by LY294002, the inhibitor of PI3K/AKT signaling pathway.Conclu-sion ActeosidepromotestheproliferationofNSCsfrom adult mice by activating PI3K/AKT pathway.
