Sanguinarine inhibits cell proliferation in MCF-7 human mammary adenocarcinoma cells
10.3969/j.issn.1001-1978.2016.06.023
- VernacularTitle:血根碱抑制人乳腺癌细胞MCF-7增殖的机制研究
- Author:
Yupan LU
;
Xianzhe DONG
;
Xia FENG
;
Yuan HU
;
Xiaoli ZHENG
;
Xiaoyue GE
;
Jinliang WANG
;
Ping LIU
- Publication Type:Journal Article
- Keywords:
SAN;
MCF-7;
ROS;
caspase;
apoptosis;
mammary adenocarcinoma;
proliferation
- From:
Chinese Pharmacological Bulletin
2016;32(6):858-862
- CountryChina
- Language:Chinese
-
Abstract:
Aim Toinvestigatewhetherexposureto Sanguinarine (SAN ) can inhibit cell proliferation in human mammary adenocarcinoma cells (MCF-7 ) and thepossiblemechanism.Methods WeexposedMCF-7 to anticancer compound SAN,cell viability was as-sessed by using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT ) reduction assay. ROS was measured using confocal microscopy,expres-sion of caspase-3 ,caspase-8 and caspase-9 were calcu-latedusingchemiluminescencemethod.Results SAN remarkably inhibited growth of human mammary adeno-carcinoma MCF-7 cells by decreasing cell proliferation. ROS release and caspase-3,caspase-8,caspase-9 ex-pression were stimulated by SAN in MCF-7 ,and these changes were abolished by the antioxidant,N-acetyl-cysteine(NAC).Conclusion Regulationofcaspases expression and release from MCF-7 cells are possibly e-voked by SAN through reactive oxygen species.
