Application of tissue spontaneous fluorescence in myocardial ischemia injury of rats
10.3969/j.issn.1001-1978.2016.06.028
- VernacularTitle:组织自发荧光在大鼠心肌缺血实验中的应用
- Author:
Fengjie LI
;
Jinyan ZHANG
;
Ping HE
;
Yikui LI
;
Le ZHAO
;
Wei HAO
;
Lianda LI
- Publication Type:Journal Article
- Keywords:
rat;
myocardial tissue;
myocardial ischemia injury;
N-BT;
autofluorescence;
in vivo imaging system;
quantitative analysis
- From:
Chinese Pharmacological Bulletin
2016;32(6):885-888
- CountryChina
- Language:Chinese
-
Abstract:
Aim Todiscusstheapplicationoftissue spontaneous fluorescence in myocardial ischemia injury of rats based on specific fluorescence detection technol-ogy.Methods Thechangeofspontaneousfluores-cence was compared between the myocardial tissue of normal rats and those of rats with myocardial ischemia injury and an quantitative analysis was then made.Re-sult Theresultsshowedthatspontaneousfluorescence of myocardial tissue for myocardial ischemia injury changed significantly.Spontaneous fluorescence signal of injury considerably was enhanced.The fluorescence signal which was quantified by FX Pro had statistical significance compared with normal myocardial tissue,P<0.01orP<0.05.Conclusion Ourresearchshows that spontaneous fluorescence of myocardial tissue can be enhanced obviously after myocardial ischemia inju-ry.Our research provides a method for the research and evaluation of myocardial ischemia injury model in rats which can be used in positioning,qualitative and quan-titative comparative study and in pathological,physio-logical,pharmacological and mechanism study.
