Early immune reconstitution after hematopoietic stem cell transplantation
10.3969/j.issn.1671-167X.2016.03.024
- VernacularTitle:造血干细胞移植后的早期免疫重建
- Author:
Mingxia ZHU
;
Wenli WAN
;
Haishen LI
;
Jing WANG
;
Yanfang WANG
;
Kai HU
;
Xiaoyan KE
- Publication Type:Journal Article
- Keywords:
Hematopoietic stem cell transplantation;
Autologous transplantation;
Allogenic transplanta-tion;
Lymphocyte subsets;
Immune reconstitution
- From:
Journal of Peking University(Health Sciences)
2016;48(3):515-522
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To search for differences in early immune reconstitution after allogenic or autolo-gous hematopoietic stem cell transplantation (HSCT).Methods:The peripheral blood (PB)from 31 adult patients undergoing allogenic HSCT (allo-HSCT,1 5 patients)or autologous HSCT (auto-HSCT, 1 6 patients)for the treatment of hematological malignancies and from 20 related healthy controls (HC) from December 201 1 to August 201 4 was used to analyze the kinetic recovery of lymphocyte subsets by means of flow cytometry during 1 2 months after HSCT.The T cell receptor rearrangement excision circle (TREC)levels among CD3 + T cells were measured in the patients and HC to evaluate the thymic-dependent T cell reconstitution.Results:The allo-and auto-HSCT recipients did not differ significantly in CD4 + T cells,CD8 na?ve T cells,effecter memory T cells (TEM),CD4 central memory T cells (TCM),mid-activated T cells and dendritic cells (DC)during the follow-up (P >0.05).But they both differed significantly from HC (P <0.05).CD8 + T cells and NK cells reconstructed rapidly.There was no significant difference in the numbers of B cells between the allo-and auto-HSCT groups from M1 to M3 (P >0.05).B cells in both the groups were lower than those in HC (P <0.05).The recovery of B cells in auto-HSCT group was faster than in allo-HSCT group at M6 and M1 2 (P <0.05).The frequen-cies of CD4 na?ve T cells and later activated T cells in allo-HSCT group were significantly higher than in auto-HSCT group at M6 and M1 2 (P <0.05).The frequencies of CD8 TCM in auto-HSCT group were significantly higher than in allo-HSCT group at M6 and M1 2 (P <0.05).The TREC levels were signifi-cantly lower than in both the groups compared with the age-matched HC during the follow-up (P <0.05).No significant difference was observed between allo-HSCT and auto-HSCT groups (P >0.05). Conclusion:The differences of the nature and the speed of lymphocyte reconstitution observed between the two patents groups were minor.This leads us to conclude that in allografted patients,immune recons-titution and subpopulations of peripheral blood lymphocytes are probably not related to the allogenicity of the graft,but due to the impaired thymus functions and slow differentiation of T lymphocytes in thymus.
