Comparison of Different Assay System for C-reactive Protein Detection

VernacularTitle:C反应蛋白测定两种检测系统间结果的比较
Author: Huaizhou WANG ; Yan CHEN ; Zhanyi YUE ; Rongrong ZHU ; Anmei DENG
Publication Type:Journal Article
Keywords: POCT; C-reactive protein; HCT; evaluation studies
From: Journal of Modern Laboratory Medicine 2016;31(3):127-130
CountryChina
Language:Chinese
Abstract: Objective To evaluate comparability of two different assay system for detecting CRP.Methods Following the profile of Clinical and Laboratory Standard Institute (CLSI)document EP9-A2,50 blood samples with anti-coagulant ED-TA-2K were collected from emergency patients at Changhai Hospital.The test result of samples by the i-CHROMA Reader was compared and evaluated with those by Beckman Immage 800.Results The linear regression equation for plasma CRP was:Y=1.076 5X-3.031 5,R2=0.986.The linear regression equation for whole blood CRP was:Y=0.882 6X-1.180 8, R2=0.931 1.For whole blood samples with low HCT (<30.45%).Used correction equation:CRP (after corrected)=CRP (before corrected)/(1-HCT).The regression equation (after corrected)was:Y=1.006 8X-3.612 2,R2=0.950 9.Con-clusion CRP concentration detected by i-CHROMA showed good correlation and comparability compared to laboratory ref-erence system by using plasma samples.Results form whole blood samples with low HCT should be corrected to improve comparability.