Discussion on the Relationship of the miR-2 1 Expression and IL-1 2 Level in Patients with Chronic Obstructive Pulmonary Disease

VernacularTitle:慢性阻塞性肺疾病患者miR-21表达与IL-12水平关系探讨*修回日期：2016-05-11
Author: Jinfang YUE
Publication Type:Journal Article
Keywords: miR-2l; IL-12; chronic obstructive pulmonary disease
From: Journal of Modern Laboratory Medicine 2016;31(3):153-155
CountryChina
Language:Chinese
Abstract: Objective To discuss the relationship of miR-21 expression and IL-12 level in patients with chronic obstructive pulmonary disease.Methods Chosed 80 patients with suspected chronic obstructive pulmonary disease(COPD)and 50 cases as control group.Real-time RT-PCR was performed to determine the expression 1 evels of miR-2 1 of peripheral blood mono-nuclear cells of three groups.To detect IL-1 2 level by ELISA method.Results There were no statistical difference among GLU,TC and TG level among stable phage,acute phage of COPD and control group(F=0.341~1.542,P>0.05).Howev-er miR-21 expression and IL-12 level had statistical difference (F=8.951~15.017,P<0.05).The expression 1evels of miR-2 1 of peripheral blood mononuclear cells of acute phage group were apparently higher than stable phage,health group(P<0.05).The concentration of IL-12 in serum of acute COPD group was higher than other two groups(P<0.05),compared with stable COPD group and health group.IL-12 level was positiveiy related to miR-21 expression (r=-0.381~0.496). Conclusion miR-2lmay be involved in the regulation of IL-12 expression.miR-21expression may be closely related to the pathogenesis of COPD.