Effect evaluation of three ELISA kits in detection of paragonimiasis
10.16250/j.32.1374.2016072
- VernacularTitle:3种ELISA试剂盒检测并殖吸虫病的效果评价
- Author:
Muxin CHEN
;
Jiaxu CHEN
- Publication Type:Journal Article
- Keywords:
Paragonimiasis;
Paragonimus westermani;
Enzyme-linked immunosorbent assay(ELISA);
Sensitivity;
Speci-ficity;
Youden index;
Effect evaluation
- From:
Chinese Journal of Schistosomiasis Control
2016;28(3):264-268
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of 3 kinds of ELISA reagents on the detection of human paragonimiasis. Meth?ods A total of 45 serum samples from patients with paragonimiasis,218 serum samples from patients with other parasitic dis?eases as well as 80 serum samples from healthy people were detected by GD?ELISA(IgG antigen ELISA detection reagent),ES?ELISA(using excretory/secretory(ES)products of Paragonimus westermani),and sAg?ELISA(using semi?purified antigen (sAg)of P. westermani),respectively. The effects of the 3 reagents were evaluated and compared. Results The sensitivities of GD?ELISA,ES?ELISA,and sAg?ELISA were 95.6%(95%CI:89.6%~100.0%),93.3%(95%CI:86.0%~100.0%)and 86.7%(95%CI:76.8%~96.6%),respectively;the specificities of the above three reagents were 88.6%(95%CI:85.0%~92.2%),88.9%(95%CI:85.3%~92.5%)and 99.0%(95%CI:97.9%~100.0%),respectively,and the Youden indexes of them were 0.84,0.82 and 0.86,respectively. Conclusion sAg?ELISA is more suitable than GD?ELISA and ES?ELISA for clin?ical sample tests in paragonimiasis endemic areas in China.
