Study on Polymorphisms and Genes Expression Levels ofα-Adducin in Patients with Coronary Artery Disease
10.3969/j.issn.1671-7414.2016.03.016
- VernacularTitle:冠心病患者α-内收蛋白基因多态性与表达水平研究
- Author:
Yangdong ZHANG
;
Jie CHEN
;
Ruixue CHU
;
Liying WANG
;
Jie YUN
;
Juan AN
;
Liang FANG
- Publication Type:Journal Article
- Keywords:
α-adducin;
coronary artery disease;
single nucleotide polymorphism;
gene expression
- From:
Journal of Modern Laboratory Medicine
2016;31(3):59-61
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the association between single nucleotide polymorphisms (SNP)and expression levels ofα-adducin(ADD1)gene in coronary artery disease (CAD)patients.Methods Extracted DNA and RNA samples of peripher-al blood white cells from 114 CAD patients and 116 healthy individuals in Jan 2011 to Oct 2013 from the General Hospital of the PLA Rocket Force.SNPs of rs3775067 and rs1263359 mutations in the ADD1 gene were analyzed with allele-specific flu-orogenic oligonucleotide probes combining hybridization.The gene expression levels were analyzed with fluorescence labeled and capillary electrophoresis technology.Results The frequencies of the genotypes and alleles of the two SNPs in the ADD1 gene were not significantly different between the two groups (χ2=0.018~1.317,all P>0.05).The ADD1 gene expression levels of CAD group (0.226±0.284)were obviously higher than that of control group (0.153±0.144,P<0.05).The gene expression levels of TC genotype of rs3775067 were obviously higher in CAD group (0.250±0.319)than that of control group (0.154±0.156,P<0.05),but the levels of the other genotypes had no significant difference between the two groups (t=0.557~1.867,all P>0.05).Conclusion The elevated ADD1 gene expression level would be risk factor for CAD.The polymorphisms of rs3775067 and rs1263359 had no relevance with CAD susceptibility.
