The experimentation about the expression of Lin28b and its clinical relevance in Pancreatic cancer
- VernacularTitle:胰腺癌中 Lin28b 表达与肿瘤关系的实验研究
- Author:
Jinheng LIU
;
Gang ZHANG
- Publication Type:Journal Article
- Keywords:
Pancreatic cancer;
Different expression cell lines of Lin28b;
RT-PCR;
Western-blot
- From:
Journal of Chinese Physician
2016;(z1):1-4
- CountryChina
- Language:Chinese
-
Abstract:
Objective Through detected the expression degree of Lin28b in three different malig-nant degree pancreatic cancer cell lines and human pancreatic cell line to study the relationship of pancreatic cancer and the express of Lin28b.Methods The RT-PCR was used to detect the expression degree of mR-NA in Lin28b in cell lines of PANC-1,BxPC-3,AsPC-1,HPC-Y5 and Western-blot was used to detect the expression degree of protein in Lin28b in cell lines of PANC-1,BxPC-3,AsPC-1,HPC-Y5,then discovering the differential expression.Results The Lin28b was high expression in three pancreatic cancer cell lines, also higher than human pancreatic cell lines,and the expression of difference had statistical significance. Conclusions The results suggested that Lin28b may play a role in the pathogenesis of pancreatic cancer, and there is a certain relationship between the expression of Lin28b and malignant degree of tumor cells.
