Effect of IGFBP7 overexpression on proliferation of human hepatocellular carcinoma HepG2 cells
- VernacularTitle:IGFBP7过表达对人肝癌细胞 HepG2增殖的影响
- Author:
Liyao WEI
;
Manyi YANG
;
Fangming WU
;
Hongjuan XU
;
Jian PENG
- Publication Type:Journal Article
- Keywords:
Insulin like growth factor binding protein 7 (IGFBP7);
Hepatocellular carcinoma;
Proliferation
- From:
Journal of Chinese Physician
2016;(z1):12-14
- CountryChina
- Language:Chinese
-
Abstract:
Objective To present study was to investigate the effects of insulin-like growth factor binding protein 7 (IGFBP7)on the proliferation of human hepatocellular carcinoma HepG2 cells.Methods Human hepatocellular carcinoma HepG2 cells was cultured,and plasmid pIRES2-ZsGreen1-IGFBP7 or empty plasmid was transfected into HepG2 cells and the cell transfection efficiency was examined by fluores-cence microscopy;MTT was performed to evaluate the effect of IGFBP7 on proliferation and apoptosis of HepG2 cells in 48 hours after transfection.Results IGFBP7 transfected group decreased cell proliferation noticeably.Conclusions Overexpression of IGFBP7 can down-regulte the proliferation of human hepatocel-lular carcinoma HepG2 cells.
