Detection of disinfectant-resistant gene inStaphylococcus aureus
10.16718/j.1009-7708.2016.03.016
- VernacularTitle:金黄色葡萄球菌耐消毒剂基因的检测
- Author:
Jianguo WU
;
Yuqing HUANG
;
Ming YAN
;
Chengwei LIU
;
Wenxiang HUANG
;
Bei JIA
- Publication Type:Journal Article
- Keywords:
Staphylococcus aureus;
disinfectant-resistant gene;
qacA/B;
mecA;
spa typing
- From:
Chinese Journal of Infection and Chemotherapy
2016;16(3):340-345
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the disinfectant-resistant geneqacA/B in the strains ofStaphylococcous aureus isolated from January 2014 to December 2014.Methods Fifty-one isolates were collected. PCR assay was used to detectmecA gene andqacA/B gene in the isolates followed byStaphylococcus protein A (spa) typing. Antimicrobial-resistant phenotypic typing was conducted to analyze the homology of theseqacA/B positive strains. The clinical information of the patients from whom the strains were isolated was collected to further understand the clinical background ofqacA/B-carryingS. aureus.Results The prevalence ofmecA and qacA/B genes was 21.6% (11/51) and 13.7% (7/51), respectively in the strains. The prevalence ofqacA/B gene in the methicillin-resistantS. aureus strains (54.5%, 6/11) was signiifcantly higher than that in the methicillin-sensitiveS. aureus strains (2.50%, 1/40). The prevalence ofmecA gene inqacA/B gene positive strains (6/7) was signiifcantly higher than that inqacA/B gene negative strains (1/7). TheseqacA/B positive strains were classiifed into 4 spa types (t037, t091, t932 and t895). The main type was t037 (4/7), which was from the pediatric ward.Conclusions The prevalence ofqacA/B gene is low in theS. aureus strains. However, the prevalence of this gene in methicillin-resistantS. aureus strains is far higher than that in methicillin-sensitiveS. aureus. spa type t037 may be a prevalent clone in pediatric ward.
