Study on the effect of influenza virus on the expression of collagen triple helix repeat containing 1
10.3969/j.issn.1006-5725.2016.10.008
- VernacularTitle:流感病毒对胶原三股螺旋重复蛋白1表达的影响
- Author:
Hui SONG
;
Fengxia XU
;
Pan ZHANG
;
Sali CHEN
;
Xinghui LIU
- Publication Type:Journal Article
- Keywords:
Influenza virus;
Collagen triple helix repeat containing 1;
Enzyme-linked immunosorbent assay;
Expression
- From:
The Journal of Practical Medicine
2016;32(10):1578-1580
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of influenza virus on the expression of collagen triple he-lix repeat containing 1 (CTHRC1). Methods A549 cells were infected with influenza virus. mRNA and protein levels of CTHRC1 were determined by RT-PCR and Western blot , CTHRC1 levels in the cell supernatants and sera of influenza virus infected patients were determined by enzyme-linked immunosorbent assay (ELISA). The difference of CTHRC1 levels between healthy controls and HCV patients was analyzed. Results Compared with controls, mRNA and protein levels of CTHRC1 were higher in A549 cells infected with H3N2. Serum CTHRC1 levels were higher in influenza virus infected patients than in healthy controls (P < 0.05). Conclusion Influenza virus can promote the synthesis and secretion of CTHRC1.
