Expression and distribution of Toll-like receptor 4 in term placentas of women with gestational diabetes ;mellitus
10.3760/cma.j.issn.1007-9408.2016.05.008
- VernacularTitle:妊娠期糖尿病孕妇足月胎盘Toll样受体4的表达分布
- Author:
Hui FENG
;
Jingmei MA
;
Huixia YANG
- Publication Type:Journal Article
- Keywords:
Diabetes,gestational;
Placenta;
Toll-like receptor 4;
Trophoblasts
- From:
Chinese Journal of Perinatal Medicine
2016;19(5):350-354
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the expression and distribution of Toll-like receptor 4 (TLR4) in term placentas of women with gestational diabetes mellitus (GDM). Methods Placental samples were collected from 36 normal full-term singleton pregnant women (control group) and 33 full-term singleton pregnant women with GDM who had elective cesarean section in Peking University First Hospital between November 2014 and June 2015. Immunohistochemical assay was used to detect the expression and distribution of TLR4 in placental tissues. T test was used to compare the expression of TLR4 in various cell types of placenta by semi-quantitative analysis. Results (1) TLR4 was expressed in the cell membrane, cytoplasm or nuclei of trophoblast, decidual cells, vascular endothelial cells and amniotic epithelial cells of term placentas. (2) Compared with the control group, the expression of TLR4 was significantly enhanced in trophoblast and decidual cells of GDM women (0.38±0.01 vs 0.31±0.01, 0.39±0.01 vs 0.34±0.01, t=5.218 and 4.525, all P<0.01). Moreover, the change of TLR4 average optical density was most significant in trophoblast. (3) There was no difference in the expression of TLR4 in vascular endothelial cells and amniotic epithelial cells in term placentas of GDM women compared with the control group (all P>0.05). Conclusions The expression of TLR4 is different in various cell types of GDM term placentas.
