The effects of exercise on the expression of synaptophysin after hypoxic-ischemic brain damage
10.3760/cma.j.issn.0254-1424.2016.05.002
- VernacularTitle:早期运动干预对脑缺血缺氧幼鼠海马区突触素蛋白表达的影响
- Author:
Yangyang GONG
;
Mei HOU
;
Aiyun YUAN
;
Xiaoping LI
;
Dawei ZHANG
- Publication Type:Journal Article
- Keywords:
Hippocampus;
Brain Injury;
Synaptophysin;
Nissl bodies;
Rats
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2016;38(5):325-328
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of early exercise on the expression of synaptophysin pro-tein and Nissl bodies in the hippocampus after hypoxic-ischemic brain damage( HIBD) , and to investigate possi-ble mechanisms. Methods A total of 35 neonatal Wistar rats aged 7 days were randomly divided into a train-ing group of 13, a control group of 11 and a sham-operation group of 11. HIBD was induced in the rats of the training and control groups, while those in the sham-operation group had the left common carotid artery separa-ted, but without ligation. Seven days after successful modeling, the training group began swimming training for 10 min every day lasting for 14 days, while the other groups were not trained. Western blotting was used to detect the expression of synaptophysin in the affected hippocampus of the brain based on the ratio of the gray band val-ues for synaptophysin and beta. Nissl staining was applied to observe the number of Nissl bodies and the morphol-ogy of the neurons in the hippocampus. Results The average expression of synaptophysin in the sinistrocere-bral hippocampus of the rats in the control group was significantly lower than that in the sham-operation group, but significantly higher than that of the training group. The control group had significantly fewer Nissl bodies than the sham-operation group, but significantly more than the training group. Conclusion Early training can in-crease the expression of synaptophysin and the number of Nissl body in the hippocampus after hypoxic-ischemic brain damage.
