Effect of different concentrations of sevoflurane on neurotoxicity of primary cultured rat cortical neurons
- VernacularTitle:不同浓度七氟醚对大鼠原代皮质神经元的影响
- Author:
Wenbo SUN
;
Limin ZHANG
;
Xiaochun ZHAO
;
Xinliao LUO
;
Lina KANG
;
Jinguang WU
;
Dongdong HUANG
;
Xiuwei SUN
- Publication Type:Journal Article
- Keywords:
Sevoflurane;
Neurotoxicity;
Apoptosis;
Cerebral cortex
- From:
The Journal of Clinical Anesthesiology
2016;32(5):480-483
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the relationship of sevoflurane neurotoxicity with the expres-sion of Bid,Bim,Puma.Methods The cortical neuron from newborn SD rat (within 24 h)were see-ded in 6 or 12 well plate,and then randomly divided into 4 groups.Rat culture cortical neurons in vitro exposed in 1%,2%,4% and 0% sevoflurane for 6h were divided into A,B,C and D group. The effect of neuron viability,death and apoptosis were assessed using CCK-8,LDH and caspase-3 cleavage 1 7kDa expression assay.The expressions of Bid,Bim and Puma were assessed by western blot.Results Compared with group D, there were significant increases of neuron death and apoptosis,but a decrease of neuron viability,and upregulated expressions of Bid,Bim and Puma in group B (P <0.05);Compared with group B,Group C had increased death and apoptosis and de-creased viability of neurons,as well as upregulated expressions of Bid,Bim and Puma (P <0.05 ). Conclusion Along with the increase of the concentration,sevoflurane neurotoxicity was increased by upregulation of Bid,Bim,Puma expression.
