Saikosaponin A attenuates cognitive function via cAMP/CREB signaling pathway in mice after traumatic brain inj ury
- VernacularTitle:柴胡皂苷 A 通过 cAMP/CREB 信号通路对脑损伤大鼠认知功能的影响
- Author:
Lieliang ZHANG
;
Jun YING
;
Fuzhou HUA
;
Zhidong ZHOU
;
Yanhui HU
;
Zhenzhong LUO
;
Guohai XU
- Publication Type:Journal Article
- Keywords:
Saikosaponin A;
Traumatic brain injury;
Cognitive function;
cAMP/CREB sig-naling pathway
- From:
The Journal of Clinical Anesthesiology
2016;32(5):484-487
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Saikosaponin A (SSA)on cognitive function and cAMP/CREB signaling pathway and expression of BDNF in mice after traumatic brain injury. Methods Sixty SD male mice were randomized into three groups:shame operation group (group S, n =20),trauma group (group T,n =20)and SSA treatment group (group A,n =20).Mice received an administration of SSA 5 mg/kg (group A)or equal volume saline (group S,group T)immediately and once daily for 5 consecutive days after trauma.The cognitive function was detected by Morris wa-ter maze test on day 1,3,7 and 14 after trauma.The hippocampal tissues were harvested after be-havioral tests and homogenized for measuring the levels of brain derived neurophic factor (BDNF)and cyclic AMP (cAMP)by ELISA as well as the levels of cAMP-response element binding protein (CREB)and phosphorylation-cAMP-response element binding protein (pCREB)by western bolt. Results Compared with group S,the escape latency and swimming distance were significantly pro-longed in group T on day 1,3,7 and 14 and group A on day 1,3 after trauma (P <0.05 );while compared with group T,they were significantly shorter in group A on day 7,14 after trauma (P <0.05).Compared with group S,the levels of BDNF,cAMP,CREB and pCREB were significantly de-creased in group T(P < 0.05 ).Compared with group T,the levels of BDNF,cAMP,CREB and pCREB were significantly increased in group A (P <0.05).Conclusion SSA can significantly improve cognitive dysfunction in mice after traumatic brain injury,and the mechanism may be related to the activation of cAMP/CREB signaling pathway and up-regulation of BDNF.
