Functional study of miRNA-301a-3p in pancreatic cancer
10.3760/cma.j.issn.1007-8118.2016.06.010
- VernacularTitle:微小RNA-301a-3p对胰腺导管腺癌细胞生物学特性的影响
- Author:
Lin ZHU
;
Kundong ZHANG
;
Chen HUANG
;
Gang CEN
;
Tao JIANG
;
Jun CAO
;
Kejian HUANG
;
Zhengjun QIU
- Publication Type:Journal Article
- Keywords:
miRNA;
Pancreatic cancer;
Oncogene
- From:
Chinese Journal of Hepatobiliary Surgery
2016;22(6):391-396
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of miRNA-301a-3p in pancreatic cancer and to correlate the expression on invasion , migration and colony formation of pancreatic cancer cells .Methods The expression of miRNA-301a-3p in 20 paired pancreatic cancer tissues and matched adjacent tissues , and pancreatic cancer cell lines and normal pancreatic ductal cells were detected by real -time PCR.miRNA-301a-3p mimics or inhibitors were used to up-regulate or down-regulate the miRNA-301a-3p level in pancre-atic cancer cell lines in order to figure out the effects of miRNA-301a-3p on cell invasion, migration and col-ony formation of pancreatic cancer cells , respectively .Results In pancreatic cancer tissues and cell lines , miRNA-301a-3p was significantly up-regulated when compared with the matched adjacent tissues ( P <0.05) and normal pancreatic ductal cells (P<0.05), respectively.Overexpression or downexpression of miRNA-301a-3p enhanced or suppressed colony formation , invasion and migration abilities of pancreatic cancer cells in vitro.Upregulation of miRNA-301a-3p promoted tumorigenesis in vivo.Conclusion miR-NA-301a-3p might function as an oncogene to promote tumorigenesis in pancreatic cancer .
