Establishment of vein bypass assisted porcine liver auto transplantation model
10.3760/cma.j.issn.1007-8118.2016.06.013
- VernacularTitle:静脉转流辅助猪自体肝移植模型的建立
- Author:
Zhiquan CHEN
;
Xiaoli FAN
;
Cheng ZENG
;
Xiaoyan HU
;
Yanfeng WANG
;
Qifa YE
- Publication Type:Journal Article
- Keywords:
Liver auto transplantation;
Bypass;
Animal model
- From:
Chinese Journal of Hepatobiliary Surgery
2016;22(6):407-411
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the vein bypass assisted porcine liver auto transplantation model and study the methods of liver cold storage during the operation , in order to provide experience for the clini-cal application of liver auto transplantation and experimental evidence for liver machine perfusion .Methods A total of 5 pigs were operated to establish the portal-vein bypass by inserting cannula into vena jugularis in-terna, femoral vein and splenic vein , with liver being hypothermic perfused and stored in situ by 4℃ UW solution through hepatic artery and portal vein .Vital signs and clinical outcomes were monitored during the operation, and the changes of liver function indexes such as aminotransferase , total bilirubin and albumin were detected at different time points .Results During the operation , the vital signs kept steady and liver were perfused well , achieving fully blood supply after reperfusion .All animals survived over 120 h.Both al-anine aminotransferase and aspartate transaminase peaked at 24 h after blood reperfusion (69.8 ±16.2 U/L and 457.7 ±50.1 U/L), and then dropped within normal range at 96 ho after reperfusion.Total bilirubin peaked at 48 h after blood reperfusion (7.1 ±3.3μmol/L), and then decreased rapidly .The plasma albu-min kept falling after blood reperfusion , reaching the minimum of 11.1 ±2.7 g/L at 4 h after reperfusion, and then rose to normal level steadily at 16 h.Conclusions The vein bypass assisted liver auto transplanta-tion could guarantee the steady vital signs and survival rate , which may supply an experimental basis for de-veloping new efficient liver preservation and restoration methods .
