Effect of cardiac shock wave therapy on human cardiac microvascular endothelial cells and its signal con-duction pathway
10.16571/j.cnki.1008-8199.2016.07.003
- VernacularTitle:心脏震波治疗对微血管 eNOS 和血管内皮生长因子的影响及其信号转导机制
- Author:
Baotong HUA
;
Ling ZHAO
;
Hongyan CAI
;
Zhiling LUO
;
Yu WANG
;
Lin LI
;
Yunzhu PENG
;
Ruijie LI
;
Tao GUO
- Publication Type:Journal Article
- Keywords:
Cardiac shock wave therapy;
Human cardiac microvascular endothelial cells;
Endothelial nitric oxide syn-thase;
Vascular endothelial growth factor;
Focal adhesion kinase inhibitor;
Calcium-activated potassium channels inhibitors
- From:
Journal of Medical Postgraduates
2016;29(7):683-687
- CountryChina
- Language:Chinese
-
Abstract:
[Abstract ] Objective Cardiac shock wave therapy (CSWT) can promote arteriogenesis in ischemic myocardia , but the mo-lecular mechanism remains unclear .The study aimed to explore the effect of CSWT on arteriogenesis in human cardiac microvascular endothelial cells ( HCMEC ) and the role of focal adhesion kinase (FAK) and Calcium-activated potassium channels (KCa) in the sig-nal conduction pathway of CSWT arteriogenesis . Methods HC-MEC cells cultured in vitro were randomly divided into control group , CSWT group , CSWT +T ( FAK inhibitor PF-573228 ) group and CSWT+F( SCa inhibitor iberiotoxin ) group.Each group received one CSWT(0.09 mJ/mm2, 200Times) 48 h after added stimulant.24 hours'conventional culture later , tests were made on the levels of endothelial nitric oxide synthase ( eNOS ) and vascular endothelial growth factor ( VEGF) mRNA as well as the changes of related protein expression . Results ①QPCR test showed that eNOS , VEGF mRNA expressions increased in CSWT group compared with control group (4.61 ±0.19 vs 3.99 ±0.17, P<0.05), while compared with CSWT group, eNOS, VEGF mRNA expressions in CSWT +T group were decreased (0.62 ±0.10 vs 0.40 ±0.02, P<0.05), eNOS, VEGF mRNA expressions in CSWT +F group were also decreased (0.53 ±0.02 vs 0.64 ±0.02, P<0.05), all the differ-ences were of statistical significance .②Western blot showed that eNOS , VEGF protein expressions increased in CSWT group compared with control group(0.63 ±0.02 vs 0.43 ±0.02, P<0.05), while compared with CSWT group , eNOS, VEGF protein expressions in CSWT+T group were decreased (0.36 ±0.01 vs 0.29 ±0.02, P<0.05), eNOS, VEGF protein expressions in CSWT +F group were also decreased (0.37 ±0.02 vs 0.30 ±0.02, P<0.05), all the differences were of statistical significance . Conclusion CSWT can improve eNOS , VEGF mRNA and protein expressions in HCMEC cells and FAK and KCa may participate in the signal conduction pathway of CSWT arteriogenesis .
