Influences different functional groups on the biological characteristics of the osteosarcoma Saos-2 cell line
10.16571/j.cnki.1008-8199.2016.07.007
- VernacularTitle:不同官能团对骨肉瘤 Saos-2细胞生物学特性的影响
- Author:
Peng HE
;
Xing ZHOU
;
Xiaozhou LIU
;
Yu CONG
;
Haidong XU
;
Gentao FAN
;
Guangxin ZHOU
;
Xin SHI
;
Sujia WU
;
Jianning ZHAO
- Publication Type:Journal Article
- Keywords:
Self-assembled monolayers;
functional group;
Osteo-sarcoma;
Saos-2 cells;
Biological characteristics
- From:
Journal of Medical Postgraduates
2016;29(7):703-707
- CountryChina
- Language:Chinese
-
Abstract:
Objective Microenvironment plays important roles in the proliferation , viability, and apoptosis of tumor cells. This study was to investigate the effects of different functional groups on the biological characteristics of the osteosarcoma Saos -2 cell line in vitro. Methods Using self-assembled monolayers of alkanethiols on gold , we prepared different terminal chemical groups , including methyl (-CH3 ) , amino (-NH2 ) , hydroxyl (-OH) , and carboxyl (-COOH ) .We determined the similar density of different functional groups by contact angle measurement and x-ray photoelectron spectroscopy , and observed the effects of different functional groups on the adhesion , proliferation, viability, and apoptosis of the osteosarcoma Saos-2 cells by fluorescence microscopy , CCK-8 as-say, flow cytometry, and scan electron microscopy (SEM). Results The surface of -COOH and -NH2 promoted the adhesion and proliferation of the of the Saos-2 cells, with a good compatibility , while that of -CH3 was unfavorable for their adhesion and proliferation and even increased their apoptosis . The promoting effects of the functional groups on the adhesion and proliferation of the cells were listed in the following order: -COOH ≥ -NH2 >-OH -CH3 , while their toxicity and apoptosis-increasing effect ranked as -CH3 -OH >-NH2 >-COOH. Conclusion The-CH3 group inhibits the adhesion and proliferation and promotes the apoptosis of Saos-2 cells, which has provided some evidence for the surface design of biomaterials.
