Metabonomics study on the intervention of Xiaoyao powder on the rates with model of liver depression and spleen deficiency patterns and liver disease
10.3760/cma.j.issn.1673-4246.2016.07.014
- VernacularTitle:逍遥散对肝郁脾虚证和肝损伤模型大鼠干预作用的代谢组学研究
- Author:
Jiatian WANG
;
Hui XIONG
;
Haiyang LIU
;
Yinan YANG
;
Lulu MU
;
Ning ZHANG
;
Jianmin LI
- Publication Type:Journal Article
- Keywords:
Xiao Yao San;
Liver qi stagnation and spleen deficiency;
Liver disease;
Metabolomics;
Gas chromatography-mass spectrometry
- From:
International Journal of Traditional Chinese Medicine
2016;38(7):624-628
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discuss the common nature of live depression and spleen qi deficiency and liver disease and the changes after the intervention ofXiaoyaopower by the metabonomics method. Methods A total of 40 rats were randomly devided into the normal control group, the liver depression and spleen qi deficiency model group, the liver depression and spleen qi deficiency treatment group, the liver disease group, the liver disease treatment group, 8 in each group. The liver depression and spleen qi deficiency model rat was established by tail-clamping infuriation and Senna gavage method, and the liver disease model rat was established by the CCl4-induced liver injury method. The rats of the liver depression and spleen qi deficiency treatment group and the liver disease treatment group were given 2 g/mlXiaoyao powder, and other groups were given the equal-volume saline. All the treatment lasted 28 days. Twelve hours after the last administration, rat metabolite profiling and contents in the serum were detected by GC-MS, the change of rat liver tissue was observed through HE staining.Results The rats of liver depression and spleen deficiency pattern and liver disease model group appeared liver cell nuclei atrophy, dissolution, degeneration necrosis and lymphocyte infiltration, and at the same time the contents of Glycine, Butanediodic acid, Propanoic acid, Methionine, Glutamine, Phenylalanine in serum were all higher than the normal control group (P<0.05). AndXiaoyao powder could reduce the content of the contents of Glycine, Butanediodic acid, Propanoic acid, Methionine,Glutamine, Phenylalanine in serum (P<0.05).ConclusionXiaoyao powder may play the role of protecting liver by the regulation of one carbon unit, sulfur containing amino acids, aromatic amino acids and amino acid of special metabolism and three tricarboxylic acid cycle.
