Inhibitory effect of EP4 antagonist on bone metastasis of prostate cancer
10.16571/j.cnki.1008-8199.2016.07.008
- VernacularTitle:EP4受体拮抗剂对前列腺癌骨转移的抑制作用
- Author:
Song XU
;
Wenquan ZHOU
;
Jingping GE
;
Zhengyu ZHANG
- Publication Type:Journal Article
- Keywords:
EP4 receptor;
Prostate cancer;
Malignant Phe-notype
- From:
Journal of Medical Postgraduates
2016;29(7):708-712
- CountryChina
- Language:Chinese
-
Abstract:
Objective The purpose of this study was to examine the inhibitory effect of ONO-AE3-208, an EP4 antagonist, on prostate cancer with bone metastasis in an animal model . Methods A PC3/LUC cell line was constructed by stably transfecting luciferin to prostate cancer PC 3 cells and inoculated into the left ventricle of nude mice to establish an animal model of prostate cancer with bone metastasis .After modeling , the animals in the experimental group and control groups were intraperitoneally given ONO -AE3-208 and double-distilled water, respectively, followed by examination of the metastasis loci and tumor burden by bioluminescence ima -ging and statistical analysis with survival curves . Results At 60 days after modeling , the animals in the control group exhibited sig-nificantly increased metastases and fluorescence burdens as compared with the experimental group (P<0.01), and the increase was in a time-dependent manner (P<0.01).At 60 days, the controls began to die while the experimental animals remained well alive , and at 180 days, the mice of the control group all died .The survival rate of the animals was significantly higher in the experimental group than in the control ( 13.3% vs 0%, P <0.01 ) and the median survival time remarkably longer in the former than in the latter group (162 d vs 116 d, P <0.01). Conclusion The EP4 antagonist ONO-AE3-208 inhibited the bone metastasis of prostate cancer and prolonged the survival time in the model mice .
