Effect of heat shock protein 72 on apoptosis of glomerulus endothelial cells in rats with hypoxic environment
10.16571/j.cnki.1008-8199.2016.07.009
- VernacularTitle:热休克蛋白72对低氧环境下大鼠肾小球内皮细胞凋亡的影响
- Author:
Jiajia YANG
;
Pengli LUO
- Publication Type:Journal Article
- Keywords:
Rat glomerulus endothelial cells;
Hypoxia;
HSP72;
Apoptosis
- From:
Journal of Medical Postgraduates
2016;29(7):713-717
- CountryChina
- Language:Chinese
-
Abstract:
Objective When the body is stimulated by hypoxia , the expression of heat shock protein 72 ( HSP72 ) is in-creased to produce anti-apoptosis effects .The aim of this paper is to study the effect of heat shock protein on apoptosis of cultured rat glomerulus endothelial cells ( GENC) under hypoxic environment . Methods Hypoxia was induced by cobalt chloride ( CoCl2 ) and GENC were divided into 5 groups ( normoxia group , hypoxia group , hypoxia+DMSO group , hypoxia+HSP72 inhibitor group , and hy-poxia+HSP72 agonist group ) according to the different intervention methods .The cell apoptosis was detected by flow cytometry and the expression of HSP72 was detected by Western blot . Results Compared with the normoxia group [(2.21 ±3.80)% and (0.23 ± 0.09)], the apoptosis rate and the expression of HSP72 were in-creased in the hypoxia group , hypoxia +DMSO group , hypoxia +HSP72 inhibitor group , and hypoxia +HSP72 agonist group [(24.54 ±3.59)% and (0.82 ±0.15), (29.25 ±1.63)% and (0.80 ±0.17), (36.07 ±1.19)%and (0.43 ±0.08), (18.10 ±2.59)%and (1.05 ±0.07)] (P<0.05).Compared with the hypoxia +DMSO group, the apoptosis rate was increased and the ex-pression of HSP72 was decreased in the hypoxia +HSP72 inhibitor group and the apoptosis rate was decreased and the expression of HSP72 was increased (P<0.05).There was no difference in the apoptosis rate and the expression between the hypoxia group and hy -poxia+DMSO group (P>0.05). Conclusion Hypoxia can induce the increased GENC apoptosis accompanied with the prolonged hypoxia .The increase or decrease of HSP 72 expression may lead to the decrease or increase of apoptosis , which is an important factor affecting the apoptosis of GENC under hypoxia .
