Determination of albiflorin in the Jinsha-Paishi infusion by HPLC

VernacularTitle:HPLC测定金沙排石冲剂中芍药苷含量
Author: Chuanyu LIU ; Jianhong TANG ; Jungang DENG
Publication Type:Journal Article
Keywords: HPLC; Jinsha-Paishi infusion; Albiflorin; Content determination
From: International Journal of Traditional Chinese Medicine 2016;38(7):633-636
CountryChina
Language:Chinese
Abstract: Objective To establish HPLC assaying methods for the determination of albiflorin in the Jinsha-Paishi infusion, and to determine the content of albiflorin of different batches ofJinsha-Paishiinfusion. Methods The chromatographic conditions were with the column of C18 (250 mm × 4.6 mm, 5μm), mobile phase of methyl alcohol-aqua (25:75,V/V), flow rate of 1.0 ml/min, and determined at wave length of 230 nm in room temperature. A total of 3 different batches of albiflorin were determined.Results In the range of 2.5-200μg, the distribution of paeoniflorin was linear (r=0.999 8), and theRSD of precision, stability and repeatability were less than 2%. The average recovery was 98.05%, andRSD was 1.65%. The contents of albiflorin in each batch were 0.080, 0.083, 0.077 mg/g.Conclusion The assaying method of HPLC is convenient, accurate and reliable to determinate the content of albiflrin in theJinsha-Paishi infusion. And the results showed that there was no difference of content of albiflorin among the different batches ofJinsha-Paishi infusion.