Clinical Usefulness of Intracytoplasmic Sperm Injection of 1-day-old Unfertilized Oocyte during IVF-ET.
- Author:
Chung Hoon KIM
;
Hyung Sik CHU
;
Eun Hee KANG
;
Hee Dong CHAE
;
Byung Moon KANG
;
Yoon Seok CHANG
- Publication Type:Original Article ; In Vitro
- Keywords:
Intracytoplasmic sperm injection (ICSI);
total fertilization failure;
conventional insemnation
- MeSH:
Embryo Transfer;
Embryonic Structures;
Family Characteristics;
Fertilization;
Fertilization in Vitro;
Humans;
Infertility;
Insemination;
Male;
Oocyte Retrieval;
Oocytes*;
Pregnancy;
Pregnancy Rate;
Semen Analysis;
Sperm Injections, Intracytoplasmic*
- From:Korean Journal of Obstetrics and Gynecology
1999;42(11):2440-2445
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: To analyze the efficacy of intracytoplasmic sperm injection (ICSI) for totally unfertilized oocytes by the conventional insemination during in vitro fertilization and embryo transfer (IVF-ET) METHODS: From March 1996 to April 1998, 15 couples who experienced total fertilization failure after conventional IVF without severe male factor infertility in semen analysis were evaluated. Fertilization were assessed by the presence of 2 pronucleus (PN) after 14-16 hours of conventional insemination. All unfertilized oocytes were reinseminated by ICSI and checked for signs of fertilization between 6-10 hours after ICSI. The embryos with fertilization and development were transferred to the uterine cavity and the outcome was analyzed. RESULTS: Total numbers of unfertilized oocytes were 120, and total numbers of oocytes injected on day 1 using ICSI were 102. Total numbers of oocytes with normal fertilization after ICSI were 74 and mean fertilization rate of 71.1 +/- 24.0% was obtained. The numbers of embryos transferred was 3.6 +/- 1.7. The biochemical pregnancy rate was 13.3% (2/15) and the clinical pregnancy rate was 6.7% (1/15) per cycle. CONCLUSION: ICSI to totally unfertilized oocytes by conventional insemination technique during IVF-ET on the next day of oocyte retrieval seems to be a relatively successful mean and afford a chance of pregnancy to the infertile couples whom the ET could not perfomed to because of total fertilization failure."
