Establishment of Model of Dynamic Change of Immune Status of ACLF Induced by ConA Repeated Administration in Mice
10.3969/j.issn.1008-7125.2016.06.002
- VernacularTitle:ConA 重复给药建立 ACLF 免疫状态动态转变的小鼠模型
- Author:
Nannan ZHANG
;
Shuyin YANG
;
Liuying CHEN
;
Shan YIN
;
Shijin WANG
;
Sanhai LIU
;
Beibei WANG
;
Zheng WANG
;
Hai LI
- Publication Type:Journal Article
- Keywords:
Acute-On-Chronic Liver Failure;
Concanavalin A;
Immune Status;
Cytokines;
Models,Animal
- From:
Chinese Journal of Gastroenterology
2016;21(6):326-330
- CountryChina
- Language:Chinese
-
Abstract:
Background:Acute-on-chronic liver failure( ACLF)is a commonly seen liver failure in China,and lacking an animal model that can effectively simulate the dynamic change of immune status of ACLF. Aims:To establish an animal model that can simulate dynamic change of immune status of ACLF by repeated administration of concanavalin A(ConA). Methods:Mice were randomly divided into normal control group and ConA repeated administration group. Mice in ConA repeated administration group were injected with ConA 15 mg/ kg through retrobulbar angular vein every 48 hours for 5 times,and mice in control group were injected with same volume of 0. 9% NaCl solution. Serum levels of IL-6,IL-10,IL- 12,TNF-α,IFN-γ,MCP-1 in peripheral blood were assessed by CBA assay,and the ratio of IL-10/ TNF-α was calculated. The expression of HLA-DR,number and proportion of CD4+ T cells and the expression of PD-1 of monocytes in peripheral blood were detected by flow cytometry. Results:Peripheral blood cytokines changed from predominated proinflammatory cytokines into predominated anti-inflammatory cytokines with the increasing in time of administration in ConA repeated administration group. Compared with control group,HLA-DR expression of monocytes in peripheral blood was significantly decreased(P <0. 05),number and proportion of CD4+ T cells were significantly decreased(P <0. 05), and PD-1 expression was significantly increased( P < 0. 05)in ConA repeated administration group. Conclusions:An animal model of ACLF immune status from systemic inflammatory response syndrome( SIRS) to compensatory antiinflammatory response syndrome(CARS)induced by repeated ConA stimulation is successfully established.
