Study on the Separation of Ketoprofen Enantiomer by Pre-column Derivation RP-HPLC
- VernacularTitle:柱前衍生化 RP-HPLC 拆分酮洛芬对映异构体的研究
- Author:
Xiaoyan LIN
;
Jieyi JIANG
;
Aili XU
;
Zhao CHEN
;
Sumei LI
- Publication Type:Journal Article
- Keywords:
Ketoprofen;
Enantiomer;
Pre-column derivation;
HPLC
- From:
China Pharmacist
2016;19(6):1045-1047
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method for the separation and determination of ketoprofen enantiomer .Methods:A pre-col-umn derivation RP-HPLC method was used with L-alanine-β-naphthylamine ( L-Ala-β-NA) as the derivation reagent .The RP-HPLC conditions were as follows: a Hypersil ODS-2 column (250 mm ×4.6 mm,5 μm) was applied, the mobile phase was acetonitrile-0.025 mol· L-1 phosphate buffer solution (40∶60, v/v) and the flow rate was 1.0 ml· min-1 , the detection wavelength was set at 245 nm and the column temperature was 25℃.The injection volume was 10μl.Results:Base line separation was achieved for the sep-aration of enantiomer from ketoprofen , and the retention time for S-(+) -ketoprofen and the R-(-) -ketoprofen was 24.2 min and 26.0 min, respectively.Dexketoprofen within the range of 0.025-0.125 mg had a good linear relationship (r=0.998 1) and the aver-age recovery was 90.93%(RSD =4.10%, n=9 ).Conclusion:The method is simple, accurate and reliable, which can be applied in the separation and determination of ketoprofen .
