Normal epithelial cell specific-1 gene hypermethylation in the carcinogenesis of hepatocellular carci-noma
10.3760/cma.j.issn.1007-8118.2016.06.012
- VernacularTitle:正常上皮细胞特异性-1基因超甲基化在肝癌发病机制中的作用
- Author:
Chao ZHOU
;
Hong CAO
;
Xuefeng RAO
;
Chuanwen LIAO
- Publication Type:Journal Article
- Keywords:
Hepatocellular carcinoma;
Gene,normal epithelial cell specific-1;
Methylation;
Cyclin
- From:
Chinese Journal of Hepatobiliary Surgery
2016;22(6):402-406
- CountryChina
- Language:Chinese
-
Abstract:
Objective To probe the gene expression of normal epithelial cell specific-1 (NES1) in normal liver cells and liver cancer cell lines , and investigate the gene methylation status and its impacts on gene expression and cell biology .Methods The expression level of NES1 mRNA was detected in HepG2 and L02 cells by RT-PCR and RT-QPCR, and the level of gene methylation was examined by MSP .We de-tected cell viability by MTT , NES1 mRNA by RT-QPCR and cyclinD1, P21 and P53 level by Western blot after treating cells with 5-Aza-CdR.Results Compared with L02, NES1 mRNA expression in HepG2 was significantly reduced, and the level of NES1 exon 3 CpG island methylation in HepG2 cells was much higher than that in L02 cells.After demethylation , NES1 mRNA expression and protein level of p 21 and p53 in HepG2 cells were up-regulated , while the cell viability and the level of CyclinD 1 were decreased .Conclu-sions In hepatocellular carcinoma , low expression of NES1 mRNA is related to the gene exon 3 CpG island methylation .NES1 exon 3 methylation may be one of the molecular mechanisms for reducing NES 1 mRNA level, and 5-aza-dC could inhibit cell proliferation by inducing cell cycle arrest in HepG 2 cells.
